陈 非 研究员/教授/博导
研究方向：
致病微生物基因组学与生物安全
学术任职：
中华医学会结核病学分会科学研究专委会常委
科研背景：
陈非博士，现为新疆医科大学省部共建中亚高发病成因与防治国家重点实验室兼职教授、结核病研究方向学科带头人、博士生导师。陈非博士从事微生物基因组学与生物安全研究工作多年，以第一或通讯作者在PNAS，JACS，Nucleic Acid Res，Clini and Transl Med (CTM), Clin Infect Dis (CID)等国际著名期刊上发表SCI论文50多篇（包括中科院1区文章10篇），总影响因子超过200，获得授权专利10余项（包括2项PCT专利）。近10年来，陈非博士及其所领导的研究团队从科技部、国家基金委及中科院等部门获得多项科研项目经费资助支持：科技部国家重点研发计划9项（含973、863及国家科技重大专项）（本人为课题负责人2项）、基金委项目9项（本人为项目负责人5项：包括国际合作项目1项、面上项目4项），中科院重点部署项目2项。
在致病微生物基因组学研究方向，陈非课题组主要聚焦于两种具有重要临床地位的下呼吸道疾病致病微生物——结核分枝杆菌和肺炎克雷伯菌的基因组学研究（据WHO报道，呼吸道感染仍是全球最致命的传染病，下呼吸道感染和结核病（TB）分别位列全球十大死亡原因的第四和第十位），与新疆医科大学呼吸科、国家CDC结核病防治中心、北京胸部肿瘤结核病医院、北京儿童医院、军事医学科学院等单位紧密合作，通过对基因组、转录组、蛋白质组、宏基因组、代谢组及表型组等多组学数据的深入挖掘、整合，系统解析上述致病菌的多种致病表型与基因型的关联，并结合多种实验手段揭示致病菌侵袭性、耐药性、持留性及宿主适应性等产生的遗传基础与分子机制(GPB, 2022; Brief Bioinform, 2021; Clini and Transl Med, 2021; Microb Biotechnol, 2021; J Glob Antimicrob Resist, 2020; Clin Infect Dis, 2019; Antimicrob Agents Chemother, 2019; Front Microbiol, 2018; Appl Environ Microbiol, 2017; Front Cell Infect Microbiol, 2017; J Biotechnol, 2017)；借助二元转录组的研究手段，系统解析上述致病菌侵染人体/免疫细胞的免疫调控网络，并揭示致病菌侵入人体后与宿主免疫细胞的相互“斗争”不同结局的免疫机制(Front Microbiol, 2019; RNA Biol, 2019; Front Microbiol, 2017 )；运用表观基因组学的研究手段，精细绘制了不同谱系结核杆菌和肺克菌全基因组精准DNA甲基化图谱，并进一步通过对基因组DNA甲基化修饰水平与表型特征的关联性解析深入探究致病菌核酸表观遗传修饰的精准调控机制(GPB, 2021, 2013; Nucleic Acids Res, 2016)；整合上述研究成果，我们为结核、肺克所引起的呼吸道感染性疾病的精准诊治提供一些新靶标，进一步的验证工作目前正在进行。
代表性文章：
1. Jia X, Yang L, Li C, Xu Y, Yang Q, Chen F*. Combining comparative genomic analysis with machine learning reveals some promising diagnostic markers to identify five common pathogenic non-tuberculous mycobacteria. Microb Biotechnol. 4(4):1539-1549, 2021.
2. Xinmiao Jia#, Cuidan Li#, Fei Chen#, Xue Li#, Peiyao Jia#, Ying Zhu#, Tianshu Sun, Fupin Hu, Xiaofeng Jiang, Yunsong Yu, Bijie Hu, Qing Yang, Mei Kang, Hongjie Liang, Kang Liao, Longhua Hu, Li Gu, Yan Jin, Qiong Duan, Shufang Zhang, Ziyong Sun, Wenxiang Huang, Hong He, Haifeng Shao, Bin Shan, Chao Zhuo, Ping Ji, Rui Zheng, Gang Li, Yingchun Xu, Qiwen Yang*. Genomic epidemiology study of Klebsiella pneumoniae causing bloodstream infections in China. Clinical and Translational Medicine, 11(11):e624, 2021 (Co-first anthor).
3. Li C, Jiang X, Yang T, Ju Y, Yin Z, Yue L, Ma G, Wang X, Jing Y, Luo X, Li S, Yang X, Chen F*, Zhou D*. Genomic epidemiology of carbapenemase-producing Klebsiella pneumoniae in china. Genomics Proteomics Bioinformatics. doi: 10.1016/j.gpb.2022.02.005， 2022.
4. Jing Fu, Ju Zhang, Li Yang, Nan Ding, Liya Yue, Xiangli Zhang, Dandan Lu, Xinmiao Jia, Cuidan Li, Chongye Guo, Zhe Yin, Xiaoyuan Jiang, Yongliang Zhao, Fei Chen*, Dongsheng Zhou*. Precision Methylome and in vivo Methylation Kinetics Characterization of Klebsiella Pneumoniae. Genomics, Proteomics & Bioinformatics, doi: 10.1016/j.gpb.2021.04.002, 2021.
5. Yu Kang, Lina Yuan, Xing Shi, Yanan Chu, Zilong He, Xinmiao Jia, Qiang Lin, Qin Ma, Jian Wang, Jingfa Xiao, Songnian Hu, Zhancheng Gao, Fei Chen*, Jun Yu*. A fine-scale map of genome-wide recombination in divergent Escherichia coli population. Brief Bioinform. 22(4):bbaa335, 2021.
6. Zhang F, Yue L, Fang X, Wang G, Li C, Sun X, Jia X, Yang J, Song J, Zhang Y, Guo C, Ma G, Sang M*, Chen F*, Wang P*. Altered gut microbiota in Parkinson’s disease patients/healthy spouses and its association with clinical features. Parkinsonism Relat Disord. 81:84-88, 2020.
7. Xia C, Zhang X, Cao T, Wang J, Li C, Yue L, Niu K, Shen Y, Ma G*, Chen F*. Hepatic Transcriptome Analysis Revealing the Molecular Pathogenesis of Type 2 Diabetes Mellitus in Zucker Diabetic Fatty Rats. Front Endocrinol. 11:565858, 2020.
8. Cuidan Li, Guannan Ma, Tingting Yang, Xiaoyu Wen, Chuan Qin, Liya Yue, Xinmiao Jia, Yicheng Shen, Dandan Lu, Lifeng Wang, Dingxia Shen*, Fei Chen*. A rare carbapenem-resistant hypervirulent K1/ ST1265 Klebsiella pneumoniae with an untypeable blaKPC-2harboured conjugative plasmid. J Glob Antimicrob Resist. 22:426-433, 2020.
9. Hairong Huang, Nan Ding, Tingting Yang, Cuidan Li, Xinmiao Jia, Guirong Wang, Jun Zhong, Ju Zhang, Guanglu Jiang, Shuqi Wang, Zhaojing Zong, Wei Jing, Yongliang Zhao, Shaofa Xu*, Fei Chen*. Cross-sectional whole-genome sequencing and epidemiological study of multidrugresistant Mycobacterium tuberculosis in China. Clin Infect Dis (CID), 69(3): 405-413, 2019.
10. Dingxia Shen, Guannan Ma, Cuidan Li, Xinmiao Jia, Chuan Qin, Tingting Yang, Lifeng Wang, Nan Ding, Liya Yue, Zhe Yin, Lijun Zeng, Yongliang Zhao, Dongsheng Zhou*, Fei Chen*. Emergence of a multidrug-resistant hypervirulent Klebsiella pneumoniae of ST23 with a rare blaCTX-M-24-harboring virulence plasmid. Antimicrob Agents Chemother (AAC), 63(3): e02273-18, 2019.
11. Ju Zhang, Xiuli Zhang, Cuidan Li, Liya Yue, Nan Ding, Tim Riordan, Li Yang, Yang Li, Charles Jen, Sen Lin, Dongsheng Zhou, Fei Chen*. Circular RNA profiling provides insights into their subcellular distribution and molecular characteristics in HepG2 cells. RNA Biology, 10.1080/15476286.2019.1565284, 2019.
12. Tingting Yang, Jun Zhong, Ju Zhang, Cuidan Li, Xia Yu, Jingfa Xiao, Xinmiao Jia, Nan Ding, Guannan Ma, Guirong Wang, Liya Yue, Qian Liang, Yongjie Sheng, Yanhong Sun, Hairong Huang* and Fei Chen*. Pan-genomic study of Mycobacterium tuberculosis reflecting the primary/secondary genes, generality/individuality, and the interconversion through copy number variations. Front Microbiol, 9: 1886, 2018.
13. Xinmiao Jia, Li Yang, Mengxing Dong, Suting Chen, Lingna Lv, Dandan Cao, Jing Fu, Tingting Yang, Ju Zhang, Xiangli Zhang, Yuanyuan Shang, Guirong Wang, Yongjie Sheng, Hairong Huang* and Fei Chen*. The Bioinformatics Analysis of Comparative Genomics of Mycobacterium tuberculosis Complex (MTBC) Provides Insight into Dissimilarities between Intraspecific Groups Differing in Host Association, Virulence, and Epitope Diversity. Front Cell Infect Microbiol, 7: 88, 2017.
14. Lingna Lv, Cuidan Li, Xiuli Zhang, Nan Ding, Tianshu Cao, Xinmiao Jia, Jinghui Wang, Liping Pan , Hongyan Jia, Zihui Li, Ju Zhang, Fei Chen* and Zongde Zhang*. RNA Profiling Analysis of the Serum Exosomes Derived from Patients with Active and Latent Mycobacterium tuberculosis Infection. Front Microbiol, 8: 1051, 2017.
15. Lei Zhang, Jun Zhong, Hao Liu, Kaiyun Xin, Chaoqiong Chen, Qiqi Li, Yahong Wei, Yao Wang, Fei Chen*, Xihui Shen*. Complete genome sequence of the drought resistance-promoting endophyte Klebsiella sp. LTGPAF-6F. J Biotechnol, 246: 36-39, 2017.
16. Yahong Wei , Jing Fu, Jianying Wu, Xinmiao Jia, Yunheng Zhou, Cuidan Li, Mengxing Dong, Shanshan Wang, Ju Zhang*, Fei Chen*. Bioinformatics analysis and characterization of highly efficient polyvinyl alcohol (PVA)-degrading enzymes from the novel PVA degrader Stenotrophomonas rhizophila QL-P4. Appl Environ Microbiol, 84 (1): e01898-17, 2017.
17. Qing Liu, Guocheng Liu, Ting Wang, Jing Fu, Rujiao Li, Linlin Song, Zhen-Gang Wang*, Baoquan Ding* and Fei Chen*. Enhanced Stability of DNA Nanostructures by Incorporation of Unnatural Base Pairs (UBPs). ChemPhysChem, 18(21): 2977-2980, 2017.
18. Fei Chen*, Yuan Zhang, Ashley B. Daugherty, Zunyi Yang, Ryan Shaw, Mengxing Dong, Steven A. Benner*. Biological phosphorylation of an Unnatural Base Pair (UBP) using a Drosophila melanogaster deoxynucleoside kinase (DmdNK) mutant. PLoS ONE, 12 (3), e0174163, 2017.
19. Lingxiang Zhu, Jun Zhong, Xinmiao Jia, Guan Liu, Yu Kang, Mengxing Dong, Xiuli Zhang, Qian Li, Liya Yue, Cuidan Li, Jing Fu, Jingfa Xiao, Jiangwei Yan, Bing Zhang, Meng Lei, Suting Chen, Lingna Lv, Baoli Zhu, Hairong Huang* and Fei Chen*. Precision methylome characterization of Mycobacterium tuberculosis complex (MTBC) using PacBio Single-Molecule Real-Time (SMRT) Technology. Nucleic Acids Res, 44(2): 730-743, 2016.
20. Fei Chen*, Mengxing Dong, Meng Ge, Lingxiang Zhu, Lufeng Ren, Guocheng Liu, and Rong Mu. The history and advances of reversible terminators used in new generations of sequencing technology. Genomics Proteomics Bioinformatics, 11(1):34-40, 2013.
21. Yang, ZY#., Chen, F#, Brian, JA., Benner, SA. Amplification, Mutation, and Sequencing of a Six-Letter Synthetic Genetic System. (2011) J Am Chem Soc. 133(38), 15105-12. (#Co-First Author).
22. Chen, F., Yang ZY, Yan MC, Brian JA, Wang GG, Benner SA. Recognition of an expanded genetic alphabet by type-II restriction endonucleases and their application to analyze polymerase fidelity. (2011) Nucleic Acid Res. 39 (9), 3949-61.
23. Chen, F., Gaucher, E. A., Leal, N. A., Hutter, D., Havemann, S. A., Govindarajan, S., Ortlund, E. A., Benner, S. A. (2010) Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection. Proc. Natl. Acad. Sci. USA. 107, 1948-1953.
24. Chen, F., Wang, R., Li, Z, Liu, B., Wang, XP., Sun, YH., Hao, DY., and Zhang, J. A novel replicating circular DNAzyme. (2004) Nucleic Acid Res, 32(8), 2336-2341.
25. Chen, F., Li, Z., Wang, R.J., Liu, B., Zeng, Z., Zhang, H.Y., Zhang, J. Inhibition of Ampicillin-resistant bacteria by novel mono- and di-DNAzymes targeted to β-lactamase mRNA. (2004) Oligonucleotides, 14(2), 80-89.
